Page 481 - ONLINE PROCEEDING BOOK WSAVA 2017
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WSVA7-0379
DSAVA: HEALTHY BREEDING
GENETIC TESTING: EXPECTATIONS, OPPORTUNITIES AND LIMITATIONS
M. Fredholm1
1University of Copenhagen, Department of Veterinary and Animal Sciences, Frederiksberg C, Denmark
GENETIC TESTING: EXPECTATIONS, OPPORTUNITIES, AND LIMITATIONS
Merete Fredholm, Professor, DVM, Ph.D., Dr.Vet.Sci.
University of Copenhagen, Faculty of Health and Medical Sciences
Groenegaardsvej 3, 1870 Frederiksberg C, Denmark mf@sund.ku.dk
Introduction
All mammals (including humans) carry a number of recessive disease genes, and disease predisposing genes which can result in the development of inherited diseases. The recessive disease genes result in disease when they occur in homozygous combinations, and
the disease predisposing genes result in disease when they occur in inappropriate constellations, or when a given individual carries too many of them. In populations with limited genetic variation the risk of developing homozygosity and/or accumulation of disease predisposing genes is higher compared to the risk in populations with large genetic variation.
The genomes of the dog breeds that we maintain today have been ‘shaped’ by two bottlenecks the last of which occurred approximately 200 years ago when the modern breeds were established through systematic breeding within closed populations. This has resulted in the es- tablishment of around 400 distinct dog breeds each with individual characteristics with respect to appearance, abilities, and behavioral predispositions. The systematic breeding within closed population, however, also implies that our dog breeds represent populations with limited genetic variation and this, in turn, requires that breeding schemes take into consideration that further reduction of genetic variation has to be avoided.
The great progress made within the area of molecular genetics during recent years has enabled researchers to study the molecular background for inherited diseases and a large number of genetic tests have been estab- lished. Genetic tests with high sensitivity and speci city can be used to circumvent loss of genetic variation; however, as discussed below, there are a number of pitfalls with genetic testing which need to be taken into consideration to avoid exclusion of perfectly healthy dogs from breeding.
Mendelian inherited diseases
According to the database Online Medelian Inheritance in Animals (OMIA: http://omia.angis.org.au/home/) there are currently 225 traits/disorders for which the key mutation is known. Many of the identi ed key mutations are mutations underlying recessive diseases. Most
of the genetic tests developed based on knowledge about the underlying mutation provide ef cient tools
both to prevent production of affected puppies and to prevent loss of genetic variation. This is accomplished by allowing breeding with carrier dogs to dogs tested free for the mutation. To exemplify this breeding strategy: In 2006 a mutation in choline O-acetyltransferase (CHAT) was shown to be the causative mutation for congenital myasthenic syndrome in old Danish pointing dogs.[1] This dog breed is a numerically small breed with a production of only around 100 puppies per year, and limited genetic variation (it was reconstructed based on a limited number of founders around 1950). All dogs used for breeding have been genotyped for the mutation until recently
and no affected puppies have been produced since the initiation of genotyping. Although the allele frequency of the disease allele was low in the population in 2006 when the mutation was detected, carrier dogs have been used for breeding. The last carrier dog was genotyped in 2011 and presently all dogs used for breeding are free due to the genotypes of parents. Thus, the mutation has been removed from the population over a 6-year period.
The large enthusiasm with respect to establishing
and using genetic testing has however, also let to introduction of tests that are insuf ciently validated. One such example is the test established for genotyping of collie eye anomaly (CEA) in, among other breeds, collies and Shetland sheepdog.[2] A study of the association between the clinical diagnosis and the genetic diagnosis in the Danish populations of the two breeds has shown that the test is only predictive in Shetland sheepdog.
[3] Thus, although the mutation originally presented by Parker and colleagues (2016) was assumed to be the causative mutation the study in the Danish populations indicates that the target of the genetic test is a markers segregating with the disease in some populations. Another example of an insuf ciently validated test is the test developed for genotyping of ichthyosis (generalized excessive scaling and hyperpigmentation) in the golden retriever.[4] A follow-up study conducted in 30 dogs which had been genotyped to be homozygous for the mutation showed that only few of the dogs had problems with dandruff.[5] This could indicate either that the disease
is in uence by other genes and/or that environmental factors play a large role in the development of the disease.
An Urban Experience
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