Page 713 - WSAVA2017
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WSVA7-0205
(CLINICAL) PATHOLOGY
CLINICAL CHEMISTRY SCREENS IN RABBITS
J. Liebscher1, P. Geppert1, E. Müller1, C. Rüffer-Girod1, C. Weber1
1Laboklin GmbH & Co. KG, Clinical Pathology, Bad Kissingen, Germany
Introduction
Pet rabbits are becoming more popular. Evaluation of lab data can aid in detection of metabolic dysfunctions and thus should be implemented in practice monitoring.
Objectives
To show that biochemistry screen can provide helpful information for monitoring of health status.
Methods
742 rabbit samples from routine submissions were included in this study. For evaluation 10 age groups were established ranging from one month to 16 years (mean of age: 5.3 years). The parameters urea, creatinine, protein, GLDH, AST, AP, γGT, CK, calcium, magnesium, phosphate, sodium and potassium were measured photometrically using cobas 8000 c702.
Results
WSVA7-0153
(CLINICAL) PATHOLOGY
RAPID MULTIPLE IMMUNOFLUORESCENT STAINING FOR DETECTION OF CYTOKERATIN AND VIMENTIN FOR USE IN DIAGNOSTIC CYTOLOGY IN CANINE NEOPLASTIC DISEASES
M. Shima-Sawa1, A. Yabuki1, M. Shiroma-Kohyama1, O. Yamato1
1Kagoshima University, Joint Faculty of Veterinary Medicine, Kagoshima, Japan
Introduction
Immunocytochemistry (ICC) is an advanced tool used in the field of veterinary diagnostic cytology. Detection of diagnostic markers, such as cytokeratin and vimentin, helps to determine if the tumor cells are of epithelial or non-epithelial origin. However, standard enzyme-based ICC has limitations in clinical usage, because it is time- consuming and often results in non-specific staining. A more convenient and reliable method is therefore needed.
Objectives
The purpose of the present study was to develop a rapid multiple immunofluorescent (RMIF) method for detection of cytokeratin and vimentin from a single cytological smear. The practical utility of the method was then evaluated in clinical samples.
Methods
Air-dried smear samples were prepared from dogs (n=13). Mouse monoclonal anti-human cytokeratin (AE1/AE3) and rabbit monoclonal anti-human vimentin (SP20) were used as primary antibodies, followed
by fluorochrome-conjugated secondary antibodies.
RMIF protocols were developed by modifying staining procedures by including a type of fixation. Immunosignals detected by RMIF-ICC were compared with those obtained in enzyme-based ICC.
Results
RMIF-ICC detected immunosignals within 45 minutes. The specificity of signals was clearly higher than that
of enzyme-based ICC. Epithelial, non-epithelial, and mesothelial cells were clearly distinguishable in a single smear of pleural effusion. In a smear of a lymph node with metastasis of epithelial tumor, the RMIF method was also able to differentiate the metastatic epithelial cells from the lymphocytes.
Conclusions
The RMIF method can be a useful tool for diagnostic cytology in veterinary medicine.
GLDH was mainly elevated in animals under 6 years whereas γGT elevations were mostly seen in animals younger than 1 or older than 6 years. 25.0% of all animals showed at least one liver parameter (AST, γGT, GLDH) above reference interval, 8.5% of these with increase in two or more of these indicating possible hepatopathy. 10.0% of the animals revealed elevated levels of both creatinine and urea indicating possible nephropathy. 2.9% of the animals showed values suspect of both, hepatopathy and nephropathy. Evidence for nutrition imbalances (risk of urinary calculi) was detected in 39.4% of the animals with calcium increase in 17.7% and lowered concentrations of phosphate in 13.7% and magnesium in 22.3%.
Conclusions
In veterinary practice biochemistry screens still are not carried out routinely. Our data show the relevance of doing so to diagnose especially changes indicating hepatopathy, nephropathy and feeding associated imbalances as early as possible.
An Urban Experience
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