Page 719 - WSAVA2017
P. 719

WSVA7-0046 DENTISTRY
Results
An Urban Experience
  DIVERSITY OF FIMBRILLIN A OF PORPHYROMONAS SPP. IN THE DENTAL PLAQUE OF LITHUANIAN DOGS
E.M. Meškytė1, I.T. Miceikienė1, M. Bratčikov2, R. Meškienė3, R. Meškys3
1Institute of Biology Systems and Genetics, Faculty of Animal Husbandry Technology- Veterinary Academy- Lithuanian University of Health Sciences, Kaunas, Lithuania
2Department of Physiology- Biochemistry- Microbiology and Laboratory Medicine, Faculty of Medicine- Vilnius University, Vilnius, Lithuania
3Department of Molecular Microbiology and Biotechnology- Institute of Biochemistry, Life Sciences Center- Vilnius University, Vilnius, Lithuania
Introduction
Periodontal diseases are one of the most common infectious diseases in dogs (affecting approximately
80% of population). Anaerobic bacteria were isolated from the periodontal pockets of dogs, in particular Porphyromonas spp.. Porphyromonas gulae and P. gingivalis are associated with periodontitis. Both P. gulae and P. gingivalis produce filmbrillin, which is known to
be one of the major factors of Porphyromonas spp. for periodontitis. The fimbria variations have an influence
on the development of periodontal diseases. P. gulae genotype B and P. gingivalis genotype II are considered the most invasive. The identification and genotyping of canine periodontal pathogens allows for a more directed approach to companion animal periodontitis pervention and treatment.
Objectives
36 oral swab probes of dogs were analysed to determined which Porphyromonas spp. and which fimA genotypes are perdominant in the dental plaque of Lithuanian dogs.
Methods
Variations of Porphyromonas spp. fimA in the dental plaque of the dogs were identified using PCR amplification and DNR sequencing and HRM-based genotyping methode.
Conclusions
Analysis of 36 speciments from the dental plaque of dogs revealed that 73,33 %, 20 % and 6,67 % of them contained Porphyromonas gulae, Porphyromonas gingivalis and both species. Phylogenetic analysis of amino acids of FimA protein revealed that 46,67 %, 26,67 %, 20 % and 6,67% of them contained P. gulae fimA A, P. gulae fimA B. P. gingivalis fimA II and mixed genotypes. The new HRM-based genotyping methode was applied and it was suitable for the Pophyromonas spp. fimA genotyping of the dental plaque of dogs.
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