Page 720 - ONLINE PROCEEDING BOOK WSAVA 2017
P. 720

An Urban Experience
WSVA7-0051 DERMATOLOGY
RNA SEQUENCING REVEALS NOVEL DIFFERENTIALLY EXPRESSED GENES IN THE SKIN OF ATOPIC AND HEALTHY STAFFORDSHIRE BULL TERRIERS
J. Anturaniemi1, S. Zaldívar-Lopez2, K. Elo3, A. Hielm-Björkman1
1Faculty of Veterinary Medicine, Department of Equine and Small Animal Medicine, University of Helsinki, Finland 2Faculty of Veterinary Medicine, Department of Genetics, University of Córdoba, Spain
3Faculty of Agriculture and Forestry, Department of Agricultural Sciences, Helsinki, Finland
Introduction
Analysis of differentially expressed genes (DEGs) in the skin of atopic dogs provides an important approach to understand the etiopathogenesis in canine atopic dermatitis (CAD). The diet is also known to have an impact on the gene expression.
Objectives
To  nd DEGs in the skin of atopic Staffordshire bull terriers using RNA-Seq, with and without the consideration of the diet.
Methods
WSVA7-0251 DERMATOLOGY
CROSS-REACTIVE CARBOHYDRATE DETERMINANT (CCD) BINDING IN SEASONAL ALLERGY TESTS IN DOGS
C. Kreil-Ouschan1, S. Mlejnek1, R. Wagner1, M. Weiss2, C. Weber2, M. Elisabeth2
1Laboklin GmbH & Co. KG, Dermatology & Allergology, Bad Kissingen, Germany
2Laboklin GmbH & Co. KG, Laboratory, Bad Kissingen, Germany
Introduction
Allergen speci c Immunglobulin E (IgE) in allergic patients is measured with the purpose of identifying the allergen(s) the pet is sensitized to. In humans some patient sera IgE has been shown to be directed against cross-reactive carbohydrate determinants (CCDs) that occur on many plant allergens. Extremely positive results in seasonal allergy tests are an indication that IgE is binding not only to proteins, but that IgE against CCDs have also been formed.
Objectives
The aim of this study was to determine if dogs in which IgE against all seasonal single allergens tested also had concomitant IgE against CCDs.
Methods
Sera from dogs for seasonal allergen-speci c IgE analysis were used. Animals in which IgE against all seasonal allergens were detected were chosen for further study
(n = 29 dogs). An Fc-Epsilon-receptor test (CHO test) was used to measure CCDs in dog sera. If this test was positive we repeated the test for seasonal allergens with a blocking solution that blocked the CCD reaction.
Results
All of the chosen samples were also positive for CCD with positive CHO tests. In all cases, repeating the allergy test for pollen with a blocking solution still showed positive results.
Conclusions
The identi cation of CCDs is indicated in extreme positive seasonal allergy test results. This study shows that the CHO test is helpful in proving that IgE antibodies against CDD occur. Blocking this binding is helpful for the selection of allergens for the immunotherapy in order to avoid the inclusion of false allergens. We present a rapid and inexpensive method.
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Skin biopsies were taken from four dogs diagnosed with CAD and four healthy dogs. Half of them were fed raw diet and half of them dry diet for a median of 139 days. The differential expression analysis was done with EdgeR and DeSeq2 algorithms and functional analyses with Ingenuity Pathway Analysis Software.
Results
Altogether 149 DEGs were found in CAD dogs compared to healthy dogs, e.g. calcyclin, ribosomal protein-L23, and transglutaminases (TGMs). Top canonical pathways in the CAD dogs were angiopoietin and epidermal growth factor signaling. When the diet was considered, 856
and 60 DEGs between CAD and healthy dogs were found in the dry and raw diet groups, respectively. In
the dry diet group, TGMs,  laggrin-2, and 23 keratins were among differentially expressed genes in CAD dogs, lipopolysaccharide/interleukin-1-mediated inhibition of the retinoid X receptors being one of the top canonical pathways. In the raw diet group e.g. angiotensinogen and TGM6 were upregulated in the CAD dogs. The top canonical pathway was γ-linolate biosynthesis II.
Conclusions
This study reports novel genes involved in CAD. The diet also has an impact on skin gene expression, which should be taken into account in future studies.
42ND WORLD SMALL ANIMAL VETERINARY ASSOCIATION CONGRESS AND FECAVA 23RD EUROCONGRESS


































































































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