Page 722 - ONLINE PROCEEDING BOOK WSAVA 2017
P. 722

An Urban Experience
WSVA7-0148 DERMATOLOGY
CORTISOL CONTENT IN HAIR MEASURED BY LC-MS/MS: A NONINVASIVE MARKER OF CHRONIC STRESS IN COMPANION ANIMALS
I. Mougeot1, J. Fish2, I. de Lannoy3, A. Grujic3, K. Cameron4, L. Mc Lean5, G. Landsberg1
1CanCog Technologies, Veterinary Clinical Research, Toronto, Canada
2Vivocore, Research, Fergus, Canada
3Intervivo Solutions, Bioanalytics, Toronto, Canada 4OntarioNutritLab, Nutrition, Fergus, Canada 5OntarioNutriLab, Nutrition, Fergus, Canada
Introduction
Serum and saliva cortisol levels are often reliable measures of the acute stress response in companion animals. However, there is scarce knowledge regarding cortisol hair content as a measure of chronic stress in pets. This study investigated baseline hair cortisol levels from healthy dogs, atopic dogs, healthy cats or cats suffering from feline idiopathic cystitis (FIC).
Objectives
Hair cortisol measures could help better monitor stress- related chronic illnesses such as atopy in dogs and FIC in cats. Measuring the cortisol hair content using Liquid chromatography-tandem mass spectrometry (LC-MS/ MS) technique could offer such a noninvasive method.
Methods
Hair was collected from 23 atopic dogs, from 13 healthy dogs, also from 8 FIC cats and from 25 healthy cats. For each animal, approximatively 150 mg of
hair was collected from three body sites. LC-MS/MS was performed on samples to quantify hair cortisol concentration. Statistical analyses were run to compare cortisol levels from healthy and atopic dogs (Mann- Whitney U test), also from healthy and FIC cats (Student t-test).
Results
The baseline hair cortisol levels were signi cantly higher (p=0.0321) in atopic [med: 4.49, min: 1.49, max: 500] than in healthy [med: 2.63, min: 1.34, max: 4.20] dogs. The baseline hair cortisol levels were not signi cantly different in healthy [mean: 2.3970, SD: 0.5225] and FIC cats [mean: 2.7386, SD: 0.8078].
Conclusions
Hair cortisol content evaluated by LC-MS/MS appears to re ect the dog’s chronic stress status when suffering from atopy. Unanticipated  ndings in cats suffering from FIC suggest that this cannot be established yet for feline subjects.
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42ND WORLD SMALL ANIMAL VETERINARY ASSOCIATION CONGRESS AND FECAVA 23RD EUROCONGRESS


































































































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