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WSVA7-0055 FELINE MEDICINE
FELINE IMMUNODEFICIENCY VIRUS (FIV) AND FELINE LEUKEMIA VIRUS (FELV) PREVALENCE IN DOMESTIC CATS FROM HUNGARY
A. Szilasi1, G. Balka1
1University of Veterinary Medicine, Pathology Department, Budapest, Hungary
Introduction
Among the retrovirus family caused diseases in domestic cats, Feline Immunodeficiency Virus (FIV) and Feline Leukemia Virus (FeLV) are the most widespread worldwide. We conducted a prevalence survey in Hungary as first in this country.
Objectives
The aim of research was to know the prevalence of these viruses among owned domestic cats in Hungary, and to analyze the genotype of isolated FIV positive strains.
Methods
EDTA-anticoagulated whole blood was collected from 184 cats presented in 13 clinics. Testing was carried
out with in-clinic ELISA test (Witness® FeLV-FIV, Zoetis), standard polymerase chain reaction (PCR). Background data was available for all cats. Data were analyzed with R software.
Results
WSVA7-0136 GASTROENTEROLOGY
HYALURONIC ACID AND TGF-B1 - USEFUL BIOMARKERS OF HEPATIC FIBROSIS?
V. Ceplecha1, K. Rehakova2, M. Skoric3, P. Proks4, J. Lorenzova1, C. Lendon5, M. Crha1
1University of Veterinary and Pharmaceutical Sciences Brno, Small Animal Clinic, Brno, Czech Republic 2University of Veterinary and Pharmaceutical Sciences Brno, Small Animal Clinical Laboratory, Brno, Czech Republic
3University of Veterinary and Pharmaceutical Sciences Brno, Department of Pathological Morphology and Parasitology, Brno, Czech Republic
4University of Veterinary and Pharmaceutical Sciences Brno, Department of Diagnostic Imaging, Brno, Czech Republic
5Edith Cowan University, Ethics- Research & Innovation, Joondalup, Australia
Introduction
Liver fibrosis is characterised as an excessive accumulation of extracellular matrix (ECM) within parenchyma observed in most types of chronic liver disorders. Hyaluronic acid (HA) - is a glycosaminoglycan component of the ECM produced by hepatic stellate cells (HSCs), in human medicine considered to be the best individual test reflecting ECM synthesis. TGF-β1 is the major mediator in fibrogenesis, also used as a marker of liver fibrosis in human medicine.
Objectives
The aim of the study was to evaluate the utility of serum HA and TGF-β1 concentrations using ELISA for the noninvasive evaluation of liver fibrosis.
Methods
Serum samples from 41 healthy dogs and 40 patients histologically diagnosed with liver disease were used. The latter group was divided into 4 subgroups: 1. vascular disorders (n=12), 2. parenchymal diseases (a. mild and significant fibrosis, n=5; b. advanced fibrosis and cirrhosis, n=5), 3. neoplasia (n=10), 4. biliary tract disorders (n=8).
Results
A Kruskal-Wallis test followed by Dunn’s post-hoc
test were performed to compare HA and TGF-β1 concentrations between groups. HA concentration was significantly increased in patients of subgroups 1 and 2b. By using cut-off values 135.94 mg/l, the sensitivity and specificity for diagnosis of advanced liver fibrosis/
24 specimens were positive to FeLV (ELISA), 30 to FIV. 24 samples showed positivity to FeLV (PCR) and 22 to FIV. Calculated true prevalence were 9,0% and 8,5% with ELISA and 8,2% and 7,3% with PCR, respectively. Correspondence between positivity and some variables was analyzed with logistic regression (p<0,05): males have higher chance to be infected with FIV, also cats with more than 3 years of age. In case of FeLV, there was no significant correspondence, however we could see the same tendencies. Phylogenetic analysis is in progress, will be done until May 2017.
Conclusions
Publications show prevalence in surrounding European countries 1,0-15,6% in case of FeLV and 2,0-30,0% in case of FIV. The higher percentages are from studies including free-roaming and shelter cats also, usually among owned cats they have lower values than ours. This shows, Hungary should make efforts on improving vaccination and screening activities.
An Urban Experience
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