Page 747 - ONLINE PROCEEDING BOOK WSAVA 2017
P. 747

WSVA7-0195
INFECTIOUS AND EMERGING DISEASES
SEROEPIDEMIOLOGICAL STUDY OF LEPTOSPIRA SPP. IN DOMESTIC CATS IN ESTONIA
A. Lehtla1, K. Must2, B. Lassen2, P. Jokelainen2, A. Viltrop2
1Estonian University of Life Sciences, Clinical Veterinary Medicine, Tartu, Estonia
2Estonian University of Life Sciences, Department of Basic Veterinary Sciences and Population, Tartu, Estonia
Introduction
Little is known about Leptospira spp. infections in domestic cats in Estonia.
Objectives
We aimed to estimate the prevalence of anti-Leptospira spp. antibodies in cats in Estonia, and to investigate whether seropositivity was associated with the lifestyle of the cats.
Methods
Sera of 551 cats were tested for antibodies against Leptospira spp. using microscopic agglutination test panel included L. interrrogans serovars Pomona, Icterohaemorragiae, Bratislava, Canicola, and Hardjo, and L.kirschneri serovar Grippotyphosa. Titres ≥ 100 were considered positive.
Results
WSVA7-0133
INFECTIOUS AND EMERGING DISEASES
DETECTION OF SALMONELLA ENTERITIDIS GROUP D1 FROM FECES OF STREET CATS IN TEHRAN, IRAN
S. MashhadyRa e1, F. Sadeghi Vafa2
1Islamic Azad University- Science and Research Branch, Small Animal Medicine and Radiology, Tehran, Iran 2Nikan Small Animal Hospital, small animal internal medicine, Tehran, Iran
Introduction
Salmonella is a genus of gram-negative bacteria of the Enterobacteriaceae family. Salmonella enterica is the type species and is further divided into different subspecies including entritidis.
Objectives
Several aims were purposed including collection information about presence of salmonellosis between street cats especially serotype information and introduction active and passive surveillance regarding public health too.
Methods
Samples obtained from 100 stray cats that were referred to small animal clinics by SPCA members from different parts of Tehran city. Routine culture and serotyping
tests were done for evaluating of genius and serovars of bacteria. Samples were cultured in 37 ˚C for 24 hours in selective media (Rambach medium). After the colonies appeared, they cultured in TSI. Results recorded after 24 hours. Positive (one case) and suspected cases(24cases) evaluated by molecular methods (PCR and M-PCR) later.
Results
Only one sample showed Salmonella Spp. in culture media that was from a 2 month kitten with hemorrhagic enteritis and severe dehydration. We found another case of Salmonella enteritidis from a female one-year-old
cat without any clinical signs by PCR. Serotyping tests showed both cases belong to groupD1 of Salmonella entritidis.
Conclusions
Although Salmonella enteritidis is an important factor in human gastroenteritis and recorded from poultry farms and products, it is supposed to detecting this kind of Salmonella may be created after exposing to related resources such as hunting birds or feeding street cats by contaminated materials or foods.
Anti-Leptospira spp. antibodies were detected in 12.9% (95% CI 10.3-15.9) of the cats. The percentage of cats that tested positive for antibodies against L. interrrogans serovars Pomona, Icterohaemorragiae, Bratislava, Canicola, and Hardjo were 9.3%, 3.5%, 2.5%, 0.4%, and 0.2%, respectively, and for antibodies against L. kirschneri serovar Grippotyphosa 7.3%. Some of the cats were positive for 2–5 different serovars. None of the cats reportedly had clinical signs of leptospirosis. The prevalence of anti-Leptospira spp. antibodies was 11.2% in pet cats: 4.4% in indoor pet cats and 17.2% in pet cats with outdoor access, and 16.3% in shelter cats. The prevalence of anti-Leptospira spp. antibodies was lower in indoor pet cats than in pet cats with outdoor access (P < 0.001).
Conclusions
More than a tenth of domestic cats in Estonia had serological evidence of exposure to Leptospira spp. The antibody prevalence was lower in indoor cats.
An Urban Experience
747


































































































   745   746   747   748   749