Page 753 - WSAVA2017
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WSVA7-0171 NEPHROLOGY
EVALUATION OF THE IMMUNE COMPLEX
IN RENAL BIOPSY OF DOGS BY IMMUNOFLUORESCENCE OF FORMALIN-FIXED AND PARAFFIN-EMBEDDED SECTION
A. Yabuki1, M. Shima-Sawa1, M. Kohyama1, O. Yamato1
1Kagoshima University, Joint Faculty of Veterinary Medicine, Kagoshima, Japan
Introduction
Renal biopsy is an essential tool for diagnosis of proteinuric kidney disease of dogs, and evaluation of the immune complex (IC) by immunofluorescence (IF) of frozen (F) sections is required for the diagnosis of IC- mediated glomerulonephritis (ICGN). However, the use of frozen sections is limited in diagnosis by renal biopsy because of certain limitations.
Objectives
We aimed to develop a reliable IF method using formalin- fixed and paraffin-embedded (FFPE) sections to detect the IC in ICGN of dogs.
WSVA7-0036 NEUROLOGY/NEUROSURGERY
RETROSPECTIVE EVALUATION OF THE PERIDURAL CATHETER THERAPY ACCORDING TO ALI-HASSAN FOR THE TREATMENT OF DISCOPATHY IN DOGS
S.E.M. Ali1, B. Martens2
1Small Animal Educational Center Merkurpark, Neurosurgery, Hamburg, Germany
2Small Animal Educational Center Merkurpark, Neurology, Hamburg, Germany
Introduction
Discopathy is the most common cause of neck or back pain in dogs. Spinal surgery has been performed routinely as treatment in such cases with variable complications.
Objectives
The purpose of the study was to elaborate the therapeutic applicability of the peridural catheter therapy (PDC) for treatment of discopathy in dogs.
Methods
During the period from 2001 to 2016 a number of 570 dogs with Hansen type I & II discs were selected for PDC therapy under CT or C-arm Fluoroscopic guidance, from a total of 4930 dogs suffering from neck or back pain presented at our center. A retrospective evaluation of the selective cases with certain criteria and fixed
time intervals up to 5 years post PDC were carried out. Signalment, history, clinical signs, imaging and owner questionnaires were reviewed.
Results
The results of the evaluation showed that 83% of the treated cases with PDC, were cured and 17% did improve or underwent another therapy. In only 5% of the treated cases a prolonged Polydipsia and Polyuria for two to four weeks were the only recorded complications.
Conclusions
In most cases PDC therapy has a clinical outcome as good as or better than decompressive surgery with little risk and it can be safely used as a minimal invasive outpatient alternative.
Methods
Renal biopsy specimens (n=12) were obtained from
dogs with protein-losing nephropathies. FFPE sections were prepared, and eight antigen retrieval pretreatment methods were set as follows: non-treatment, digestion with trypsin (Try), microwave (MW) heating in citrate buffer (MW-CB; pH 6.0), MW in tris-EDTA buffer (MW-TE; pH 9.0), and combinations of the above. Then, the presence of IgG, IgA, IgM, and C3 were detected with Alexa Fluor 488-conjugated secondary antibodies.
Results
Combination of Try (incubation for 30 min; Try-30) and MW-TE was the most effective for diagnosis, with clear positive signals being obtained for IgG, IgA, IgM, and C3 by IF-FFPE in all the samples. Granular signals, an important diagnostic indicator of ICGN, were clearly observed not only by IF-F but also by IF-FFPE after combined pretreatment with Try-30 and MW-TE. Signals for immunoglobulins and C3 by standard IF-F and the present IF-FFPE were almost completely matched in all samples.
Conclusions
The present IF-FFPE method can be used as an efficient tool in renal biopsy for diagnosis of proteinuric kidney disease of dogs.
An Urban Experience
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