Page 766 - ONLINE PROCEEDING BOOK WSAVA 2017
P. 766

An Urban Experience
WSVA7-0007 ORTHOPEDICS
THE TREATMENT OF PLATELET-RICH PLASMA INJECTIONS FOR DOGS WITH OSTEOARTHRITIS AND THE RESULTS OF MMP- 2,9 AND CYTOKINES PROFILES
M. Arican1, A. Simsek2, K. Parlak1, K. Atlı2, G. Sönmez3
1University of Selcuk - Faculty of Veterinary Medicine, Department of Surgery, Konya, Turkey
2University of Selcuk - Faculty of Veterinary Medicine, Department of Virology, Konya, Turkey
3University of Selcuk - Faculty of Veterinary Medicine, Department of Genetics, Konya, Turkey
Introduction
Plazma rich platelet may have a favorable effect on OA.
Objectives
The study was to determine the levels of MMP-2 and 9, IL-1a, IL-6, IL-10,TNF-a and PGE2 in the synovial  uids after intra-articular injection of an autologous PRP for the treatment of osteoarthritis in dogs.
WSVA7-0008 ORTHOPEDICS
CLINICAL OUTCOME OF TTA-RAPID IN DOGS WITH CRANIAL CRUCIATE LIGAMENT RUPTURE
M. Arican1, K. Parlak1, H. Sahin1, G. Sönmez2
1University of Selcuk - Faculty of Veterinary Medicine, Department of Surgery, Konya, Turkey
2University of Selcuk - Faculty of Veterinary Medicine, Department of Genetics, Konya, Turkey
Introduction
Rupture of the cranial cruciate ligament (CCL) is a common problem in dogs.
Objectives
The study was intended to apply the modi ed (TTA)- Rapid methods in dogs with CCL and report clinical outcome, complications and synovial  uids analysis.
Methods
The study materials was consisted of different breeds, male, average weight of 32 kg and 17 dogs between the ages of 2-8 which were obtained with lameness in the hind limbs by primarily systemic and clinical examination. Radiographs of the affected joint obtained at weeks 0, 4, 8, 12 were examined. TTA-rapid surgery was followed by (Samoy et al 2015. Lameness and pain were assessed by Hudson Visual Analog Scale and Canine Brief Pain Inventory. Synovial  uid gelatinase activity levels were determined by gelatin zymography.
Results
Artroscopic examination and arthrotomy was shown no meniscus damage. On clinical examination at 3 months, 8 dogs (41.1%) had an excellent outcome,6 dogs (35.2%) had a good outcome, and 3 dogs (17.6%) had a moderate outcome. Metalloproteinases activity were increased of 70 kDa enzyme in pre-operatif treatment synovial  uids. Enzyme activity was also seen at 204 kDa (MMP-9 pro-dimer) and 257 kDa (MMP-9 dimer). 100 kDa (MMP-9) band was totally inhibited within 60th day in some cases. The 70 kDa enzyme (MMP-2) was partially inhibited.
Conclusions
TTA-Rapid, relatively non-invasive soft tissue approach. Synovial  uids analysis for gelatinases were shown encourage results to decreased MMP 2 and MMP-9 activity after arthrocentesis in TTA-rapid group. Further studies must be needed to determine if the results remain the same in larger groups.
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Methods
Twenty dogs were used as a materials. 14 dogs were used as osteoarthritis groups and 6 dogs were used as a control. The dogs were randomly assigned to a tretment or control group. Double centrifuged method will be used to obtain platelet-rich plasma. Lameness, pain assesment and arthrocentesis were done at days 0,1,3,5,7, 15 and week 4, 8 and 12.
Results
MMP-2 and 9 enzyme bands were inhibited after PRP treatment group in 30 days following injection and there were no change in saline (0.9 % NaCl) injected group. The TNF-a had observed a slight decline during the  rst week. In the present study the pro-in ammatory IL-6 levels remained higher in the treatment group. IL-1b levels was slight decreased while TNF-a levels was decreased in 5th days after PRP injection. In IL-6 and IL- 10 levels increased with 60 and 90.days.
Conclusions
PRP applications inhibited MMPs activity and some in ammatory parameters in synovial  uids. This can reduce the signs and symptoms of in ammatory conditions. Cytokines levels are decreased within a week after intra- articular injection. Therefore, PRP should be injected several times at regular intervals instead of a single application. PRP is an effective and safe method for treatment of OA. This work was supported by TUBITAK (Proj. No. 2130175)
42ND WORLD SMALL ANIMAL VETERINARY ASSOCIATION CONGRESS AND FECAVA 23RD EUROCONGRESS


































































































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