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WSVA7-0228 ORTHOPEDICS
EFFECT OF DEMINERALIZATION OF CANINE CORTICAL BONE ON OSTEOGENIC DIFFERENTIATION OF ADIPOSE DERIVED MESENCHYMAL STROMAL CELLS
K.R. Jo1, Y. Kim1, S. Lee1, Y. Yoon1, W. Kim1, O. Kweon1
1College of Veterinary Medicine - Seoul National University, Surgery, Seoul, Republic of Korea
Introduction
Demineralized allografts and mesenchymal stromal cells have been used to promote bone regeneration. However, degree of demineralization of cortical bone for combination therapy with adipose derived mesenchymal stromal cells (ad-MSCs) has not been clarified.
Objectives
In vitro osteogenic ability of ad-MSCs on allografts was investigated in relation to the degree of demineralization.
Methods
It was divided into 3 groups according to the degrees of demineralization by 0.6 N hydrochloric acid; partially demineralized bone for 12 h, PDB; fully demineralized bone for 48 h, FDB; non- demineralized bone, NDB
as control. Grafts were prepared as a disc type in the diameter of 6 mm for in vitro evaluation. The degree
of demineralization and structure of allografts were evaluated by micro-computed tomography (CT) and scanning electron microscope (SEM). The adhesion and proliferation rates of ad-MSCs were measured by MTS assay.
Results
WSVA7-0194 ORTHOPEDICS
SYNOVIAL FLUID AND BIOMARKERS
CRP (C-REACTIVE PROTEIN) AND COMP (CARTILAGE OLIGOMERIC MATRIX PROTEIN) ANALYSIS IMPORTANCE IN DIAGNOSTIC OF OSTEOARTHRITIS IN CANINES
R. Noreikaite-Bulotiene1, J. Sengaut1, V. Bizokas2
1Small animal clinic, Surgery department, Vilnius, Lithuania
2Small animal clinic, Surgery department, Kaunas, Lithuania
Introduction
Osteoarthritis (OA) can be considered as a primary or secondary cause of cranial cruciate ligament rupture (CCLR) in canines. While research has so far been focused on OA biomarkers that can provide information about joint changes prior to the development of late stage of degenerative processes, none of this is currently in use in clinical practice.
Objectives
The aim of our study was to evaluate changes in canine synovial fluid physical properties, cells quantity and composition from synovial fluid smear.
Methods
The concentration of biomarkers CRP and COMP in plasma and synovial fluid were evaluated using ELISA kits. Samples of synovial fluid was taken from two groups of Labrador retrievers. While the first group consisted of patients in which the cranial cruciate ligament rupture has been diagnosed (n=30), the second (control) group was composed of clinically healthy patients (n=10).
Results
In control group, the samples of synovial fluid were
of yellow-grey color, medium viscosity, not turbid and tested positive for the mucin clot test. The majority of the canine synovial fluid samples from the first group displayed a yellow-grey color, was non-viscous while the mucin clot test was negative and without turbidity. In the same group we observed an increase of neutrophil and mononuclear cells, as well as of biomarkers CRP and COMP from plasma and synovial fluid.
Conclusions
It was shown that synovial fluid analysis is a particularly important diagnostic tool, especially when there is
no clinical evidence of OA, and could be valuable for detection of early degenerative changes in the cartilage.
Osteogenic related genes were assessed by real-time PCR. Demineralization rates of PDB and FDB were 57.13 % and 92.30 %, respectively. Adhesion rates of ad-MSCs on the NDB, PDB and FDB, respectively, were 53.41 %, 60.65 % and 61.32 %. Proliferation rate of ad-MSCs on FDB from 2 days after culture increased significantly than other groups (P<0.05). Osteogenic genes of ALP, BMP-7 and TGF- β in FDB group at the day 3 of culture also elevated significantly as compared to other groups.
Conclusions
It was suggested that fully demineralized bone might be a suitable scaffold for biocompatability and osteogenic differentiation of ad-MSCs in the repair of bone defects.
An Urban Experience
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