Page 775 - WSAVA2017
P. 775

WSVA7-0154 OTHER
EVALUATION OF CLINOFIBRATE EFFECT ON CANINE PLASMA TRIGLYCERIDE USING EPIDEMIOLOGICAL ANALYSIS
Y. Sato1, N. Arai2, H. Yasuda3, Y. Mizoguchi1
1Meiji University, Agriculture, Kawasaki, Japan 2Spectrum Lab Japan, Veterinary Research and Development, Meguro-ku, Japan
3Yasuda Veterinary Clinic, Veterinary, Meguro-ku, Japan
Introduction
Hypertriglyceridemia develops in Canis lupus familiaris due to lack of exercise, excessive caloric intake,
and genetic factors. Clinofibrate is a drug for human hypertriglyceridemia, but in Japan it is now also prescribed for dogs even though there is little knowledge about the medicinal effect on these animals.
Objectives
Our objective was to evaluate the effect of Clinofibrate on the level of plasma triglyceride (TG) in dogs.
Methods
We collected TG concentration data of 306 dogs from Spectrum Lab Japan that met the following conditions: (1) Data were measured after fasting more than 8 hours, (2) Presence of data before and after first medication, and (3) Two measurements were performed within one year. Samples were categorized into breed groups, comprising the top five breeds plus a composite group containing the other 30 breeds. The samples were
also categorized into two groups according to the presence or absence of drug efficacy. Logistic analysis was performed with regard to some dog characteristics (breed, sex, neuter status, BCS, and age).
Results
There was a decrease in TG content after drug treatment, with an average reduction of 53.70%. However, drug refractory occurred in 17.65% of the dogs. Logistic analysis showed no relationship between dog characteristics and drug efficacy.
Conclusions
We confirmed that Clinofibrate could effectively reduce TG in dogs with hypertriglyceridemia. The lack of any relationship between drug efficacy or drug refractory and dog characteristics, indicates effect of rare gene variations in lipid metabolism that might have inhibited the drug’s action.
WSVA7-0174 REPRODUCTION, PEDIATRICS
USE OF DOCOSAHEXAENOIC ACID TO PREVENT ACROSSOMAL AND SPERM MEMBRANE INTEGRITY IN DOMESTIC CAT COOLED SPERMATOZOA – PRELIMINARY RESULTS.
A. Camila Louise1, S. Juliana de Almeida1,
D. Camila de Paula Freitas1, C. Laíza Sartori1, S.J. Edjalma Rodrigues1, L. Maria Denise1
1UNESP, Department of Animal Reproduction and Veterinary Radiology, Botucatu, Brazil
Introduction
Cryopreservation of semen is an important tool for semen conservation; however damage to spermatozoa and acrosomal membrane may occur due oxidative stress. To overcome this issue, the addition of antioxidants prior to cryopreservation has been used (1,2).
Objectives
We aimed to evaluate integrity of sperm and acrosome membrane from domestic cat cooled semen extended with or without addition of antioxidant.
Methods
Six semen samples were collected (three from cat A,
two from B; one from C) using artificial vagina technique. Semen was divided in 2 aliquots and each one was diluted. BB: Botubov® (Botupharma, Botucatu, Brazil) and BB4: Botubov® add with docosahexaenoic acid (0.04mg); to a final sperm concentration of 40 x 106/mL and cooled at 5oC for 48 h. For sperm and acrossomal membrane integrity an association of propidium iodide (PI; P4170, Sigma Aldrich), FITC-PSA (L0770, Sigma Aldrich) and Hoechst 33342 (14533, Sigma Aldrich), was performed using a C11- BODYPY (D-3861, Molecular Probes Inc., Eugene, Oregon, USA)using flow cytometry. Normality test was performed using Kolmogorov-Smirnov (K-S), ANOVA followed by Tukey using GraphPad Prism 6.
Results
Results are described in Table 1.
An Urban Experience
    775
                   

































































   773   774   775   776   777